Key background papers from the team

Doherty, C.P.A., Ulamec, S., Maya-Martinez, R., Good, S.C., Makepeace, J., Khan, N., van Oosten-Hawle, P., Radford, S.E. & Brockwell, D.J. (2020) A short motif in the N-terminal region of α-synuclein is critical for both aggregation and function. Nat. Struct. Mol. Biol. 27, 249-259.

Horne, J.E., Walko, M., Calabrese, A.N., Levenstein, M.A., Brockwell, D.J., Kapur, N., Wilson, A.J. & Radford, S.E. (2018) Rapid mapping of protein interactions using tag-transfer photocrosslinkers. Angew. Chemie. Int. Ed. 57, 16688-16692.

Calabrese, A.N.,  Schiffrin, B., Watson, M., Karamanos, T.K., Walko, M., Humes, J.R., Horne, J.E., White, P., Wilson, A.J., Kalli, A.C., Tuma, R., Ashcrift, A.E., Brockwell, D.J. & Radford, S.E. (2020) Inter-domain dynamics in the chaperone SurA permit multi-site binding to its outer membrane protein clients. Nat. Comms11, 2155.

Ochi, T., Quarantotti, V., Lin, H., Jullien, J., Rosa e Silva, I., Boselli, F., Barnabas, D., Johnson, C.M., McLaughlin, S.H., Freund, S.M.V., Blackford, A.N., Kimata, Y., Goldstein, R.E., Jackson, S.P., Blundell, T.L., Dutcher, S.K., Gergely, F. & van Breugel, M. (2020) CCDC61/VFL3 is a paralog of SAS6 and promotes ciliary functions. Structure 28, 674-689.

Ochi, T., Blackford, A.N., Coates, J., Jhujh, S., Mehmood, S., Tamura, N., Travers, J., Wu, Q., Draviam, V.M., Robinson, C.V., Blundell, T.L & Jackson, S.P. (2015) PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair. Science 347, 185-188.

Burgess, S.G., Mukherjee, M., Sabir, S., Joseph, N., Gutiérrez-Caballero, C., Richards, M.W., Huguenin-Dezot, N., Chin, J.W., Kennedy, E.J., Pfuhl, M., Royale, S.J., Gergely, F. & Bayliss, R. (2018) Mitotic spindle association of TACC3 requires Aurora-A-dependent stabilization of a cryptic α-helix. EMBO J. 37 (8), doi: 10.15252/embj.201797902

Richards, M.W., Burgess, S.G., Poon, E., Carstensen, A., Eilers, M., Chesler, L. & Bayliss, R. (2016) Structural basis of N-Myc binding by Aurora-A and its destabilization by kinase inhibitors. Proc. Natl. Acad. Sci. USA.113, 13726-13731.

Cuvertino, S., Hartill, V., Colyler, A., Garner, T., Nair, N., Al-Gazali, L., Canham, N., Faundes, V., Flinter, F., Hertercant, J., Holder-Espinasse, Jackson, B., Lynch, S.A., Nadat, F., Narasimhan, V.M., Peckham, M., Sellers, R., Seri, M., Montanari, F., Southgate, L., Squeo, G.M., Trembath, R., van Heel, D., Ventuno, S., Weisberg, D., Stals, K., Ellard, S., Genomics England, Research Consortium, Barton, A., Kimber, S.J., Sheridan, E., Merla, G., Stevens, A., Johnson, C.A. & Banka, S. (2020) A restricted spectrum of missense KMT2D variants cause a multiple malformations disorder distinct from Kabuki syndrome. Genet Med. 22, 867-877.

Johnson, C.A. & Malicki, J.J. (2019) The nuclear arsenal of cilia. Dev Cell 49, 161-170.

Tiede, C., et al. (2017), Bedford, R., Heseltine, S.J., Smith, G., Wijetunga, I., Ross, R., AlQallaf, D., Roberts, A.P.E., Balls, A., Curd, A., Hughes, R.E., Martin, H., Needham, S.R., Zanetti-Domingues, L.C., Sadigh, Y., Peacock, T.P., Tang, A.A., Gibson, N., Kyle, H., Platt, G., Ingram, N., Taylor, T., Coletta, L.P., Manfield, I., Knowles, I., Bell, S., Esteves, F., Maqbool, A., Prasad, R.K., Drinkhill, M., Bon, R.S., Patel, V., Goodchild, S.A., Martin-Fernandez, M, Owens, R.J., Nettleship, J.E., Webb, M.E., Harrison, M., Lippiat, J.D., Ponnambalam, S., Peckham, M., Smith, A., Ko Ferrigno, P., Johnson, M., McPherson, M.J. & Tomlinson, D.C. (2017) Affimer proteins are versatile and renewable affinity reagents. Elife 27 (6), doi.org/10.7554/eLife.24903.001

Hughes, D.J., Tiede,C., Penswick, N., Ah-San Tang, A., Trinh, C.H., Mandal, U., Zajac, K.Z., Gaule, T., Howell, G., Edwards, T.E., Duan, J., Feyfant, E., McPherson, M.J., Tomlinson, D.C., Whitehouse, A. (2017) Generation of specific inhibitors of SUMO-1– and SUMO-2/3–mediated protein-protein interactions using Affimer (Adhiron) technology. Sci. Signal. 10 (505), doi: 10.1126/scisignal.aaj2005

Wright, M.H., Fetzer, C.F. & Sieber, S.A. (2017) Chemical Probes Unravel an Antimicrobial Defense Response Triggered by Binding of the Human Opioid Dynorphin to a Bacterial Sensor Kinase J. Am. Chem. Soc. 139, 6152-6159.

Wright, M.H. (2018) Chemical proteomics of host‐microbe interactions. Proteomics 18 (18), DOI:10.1002/pmic.201700333

Ibarra, A., Bartlett, G.J., Hegedüs, Z., Dutt, S., Hobor, F., Horner, K.A., Hetherington, K., Spence, K., Nelson, A., Edward, T.A., Woolfson, T.A., Sessions, R.B., & Wilson, A.J.(2019) Predicting and Experimentally Validating Hot-spot Residues at Protein-Protein Interfaces. ACS Chem. Biol., 14 (10), 2252-2263.

Grison, M., Burslem, G., Miles, J.A., Pilsl, L.K.A., Yeo, D.J., Imani, Z., Warriner, S.L., Webb, M.E. & Wilson, A.J. (2017) Double Quick, Double Click Reversible Peptide “Stapling”. Chem. Sci., 7, 5166-5171.

Quarantotti, V., Chen, J.X., Tischer, J., Gonzalez Tejedo, C., Papachristou, E., D’Santos, C.S., Kilmartin, J.V., Miller, M.L. & Gergely, F. (2019) Centriolar satellites are acentriolar assemblies of centrosomal proteins. EMBO J  38, doi/full/10.15252/embj.2018101082.

Joseph, N., Al-Jassar, C., Johnson, C.M., Andreeva, A., Barnabas, D.D., Freund, S.M.V., Gergely, F. & van Breugel, M. (2018) Disease-associated mutations in CEP120 destabilise the protein and impair ciliogenesis. Cell Rep.  23 (9), 2805-2818.

Chavali, P.L., Chandrasekaran, G., Barr, A.R., Tatrai, P., Taylor, C., Papachristou, E.K., Woods, C.G., Chavali, S. & Gergely, F. (2016) A CEP215-HSET complex links centrosomes with spindle poles and drives centrosome clustering in cancer. Nat Commun. 11005, doi.org/10.1038/ncomms11005

Burgess, S.G., Peset, I., Joseph, N., Cavazza,T., Vernos, I., Pfuhl, M., Gergely, F. & Bayliss, R. (2015) Aurora-A-dependent control of TACC3 influences the rate of mitotic spindle assembly. PLoS Genet. 11(7), doi.org/10.1371/journal.pgen.1005345